Do It Fast: Immediate Functional Testing of Membrane Pumps Expressed into Nanodiscs (Biophysical Journal)

Functional analysis of a large number of ion pumps and their variants is a time-consuming task and often retards research progress of this important class of proteins. The modus operandi is to heterologously overexpress the proteins into single-celled bacteria, yeast, or cell cultures and to purify by extraction from the membrane environment with the help of detergents and subsequent purification steps. The proteins are then subjected to biophysical investigations. In their recent work, Henrich et al.