MyJournals Home  

RSS FeedsIJMS, Vol. 18, Pages 2024: Role of the Genes of Type VI Secretion System in Virulence of Rice Bacterial Brown Stripe Pathogen Acidovorax avenae subsp. avenae Strain RS-2 (International Journal of Molecular Sciences)

 
 

21 september 2017 15:15:55

 
IJMS, Vol. 18, Pages 2024: Role of the Genes of Type VI Secretion System in Virulence of Rice Bacterial Brown Stripe Pathogen Acidovorax avenae subsp. avenae Strain RS-2 (International Journal of Molecular Sciences)
 


The Type VI secretion system (T6SS) is a class of macromolecular machine that is required for the virulence of gram-negative bacteria. However, it is still not clear what the role of T6SS in the virulence of rice bacterial brown stripe pathogen Acidovorax avenae subsp. avenae (Aaa) is. The aim of the current study was to investigate the contribution of T6SS in Aaa strain RS2 virulence using insertional deletion mutation and complementation approaches. This strain produced weak virulence but contains a complete T6SS gene cluster based on a genome-wide analysis. Here we compared the virulence-related phenotypes between the wild-type (RS-2) and 25 T6SS mutants, which were constructed using homologous recombination methods. The mutation of 15 T6SS genes significantly reduced bacterial virulence and the secretion of Hcp protein. Additionally, the complemented 7 mutations ?pppA, ?clpB, ?hcp, ?dotU, ?icmF, ?impJ, and ?impM caused similar virulence characteristics as RS-2. Moreover, the mutant ?pppA, ?clpB, ?icmF, ?impJ and ?impM genes caused by a 38.3~56.4% reduction in biofilm formation while the mutants ?pppA, ?clpB, ?icmF and ?hcp resulted in a 37.5~44.6% reduction in motility. All together, these results demonstrate that T6SS play vital roles in the virulence of strain RS-2, which may be partially attributed to the reductions in Hcp secretion, biofilm formation and motility. However, differences in virulence between strain RS-1 and RS-2 suggest that other factors may also be involved in the virulence of Aaa.


 
127 viewsCategory: Biochemistry, Biophysics, Molecular Biology
 
IJMS, Vol. 18, Pages 2010: A Comprehensive Survey of the Roles of Highly Disordered Proteins in Type 2 Diabetes (International Journal of Molecular Sciences)
IJMS, Vol. 18, Pages 2029: Targeted Delivery of siRNA with pH-Responsive Hybrid Gold Nanostars for Cancer Treatment (International Journal of Molecular Sciences)
 
 
blog comments powered by Disqus


MyJournals.org
The latest issues of all your favorite science journals on one page

Username:
Password:

Register | Retrieve

Search:

Molecular Biology


Copyright © 2008 - 2024 Indigonet Services B.V.. Contact: Tim Hulsen. Read here our privacy notice.
Other websites of Indigonet Services B.V.: Nieuws Vacatures News Tweets Nachrichten