Near-Atomic Resolution Structure Determination in Over-Focus with Volta Phase Plate by Cs-Corrected Cryo-EM (Structure)

Fan et al. took a unique advantage of the optical system by combining the Volta phase plate and Cs-corrector in a modern TEM to collect high-resolution micrographs of frozen-hydrated apo-ferritin in over-focus imaging conditions and determine the structure of apo-ferritin at 3.0 Å resolution.