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RSS FeedsSensors, Vol. 18, Pages 1616: SPR Biosensors in Direct Molecular Fishing: Implications for Protein Interactomics (Sensors)

 
 

21 may 2018 06:01:44

 
Sensors, Vol. 18, Pages 1616: SPR Biosensors in Direct Molecular Fishing: Implications for Protein Interactomics (Sensors)
 


We have developed an original experimental approach based on the use of surface plasmon resonance (SPR) biosensors, applicable for investigation of potential partners involved in protein–protein interactions (PPI) as well as protein–peptide or protein–small molecule interactions. It is based on combining a SPR biosensor, size exclusion chromatography (SEC), mass spectrometric identification of proteins (LC-MS/MS) and direct molecular fishing employing principles of affinity chromatography for isolation of potential partner proteins from the total lysate of biological samples using immobilized target proteins (or small non-peptide compounds) as ligands. Applicability of this approach has been demonstrated within the frame of the Human Proteome Project (HPP) and PPI regulation by a small non-peptide biologically active compound, isatin.


 
62 viewsCategory: Chemistry, Physics
 
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