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RSS FeedsIJMS, Vol. 19, Pages 1557: Supplementation with IL-6 and Muscle Cell Culture Conditioned Media Enhances Myogenic Differentiation of Adipose Tissue-Derived Stem Cells through STAT3 Activation (International Journal of Molecular Sciences)

 
 

24 may 2018 18:00:37

 
IJMS, Vol. 19, Pages 1557: Supplementation with IL-6 and Muscle Cell Culture Conditioned Media Enhances Myogenic Differentiation of Adipose Tissue-Derived Stem Cells through STAT3 Activation (International Journal of Molecular Sciences)
 


Mature skeletal muscle cells cannot be expanded in culture systems. Therefore, it is difficult to construct an in vitro model for muscle diseases. To establish an efficient protocol for myogenic differentiation of human adipose tissue-derived stem cells (hADSCs), we investigated whether addition of IL-6 and/or myocyte-conditioned media (CM) to conventional differentiation media can shorten the differentiation period. hADSCs were differentiated to myocytes using the conventional protocol or modified with the addition of 25 pg/mL IL-6 and/or C2C12 CM (25% v/v). The expression of MyoD and myogenine mRNA was significantly higher at 5–6 days after differentiation using the modified protocol than with the conventional protocol. mRNA and protein expression of myosin heavy chain, a marker of myotubes, was significantly upregulated at 28 and 42 days of differentiation using the modified protocol, and the level achieved after a 4-week differentiation period was similar to that achieved at 6 weeks using the conventional protocol. The expression of p-STAT3 was significantly increased when the modified protocol was used. Similarly, addition of colivelin, a STAT3 activator, instead of IL-6 and C2C12 CM, promoted the myogenic differentiation of ADSCs. The modified protocol improved differentiation efficiency and reduced the time required for differentiation of myocytes. It might be helpful to save cost and time when preparing myocytes for cell therapies and drug discovery.


 
61 viewsCategory: Biochemistry, Biophysics, Molecular Biology
 
IJMS, Vol. 19, Pages 1558: Pannexin-1 in Human Lymphatic Endothelial Cells Regulates Lymphangiogenesis (International Journal of Molecular Sciences)
IJMS, Vol. 19, Pages 1556: Isoviolanthin Extracted from Dendrobium officinale Reverses TGF-?1-Mediated Epithelial-Mesenchymal Transition in Hepatocellular Carcinoma Cells via Deactivating the TGF-?/Smad and PI3K/Akt/mTOR Signaling Pathways (International Journal of Molecular Sciences)
 
 
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