Viruses, Vol. 10, Pages 275: Live-Cell Imaging of Early Steps of Single HIV-1 Infection (Viruses)

Live-cell imaging of single HIV-1 item offers a unique opportunity to delineate the spatio-temporal regulation of infection. Novel virus labeling and imaging approaches enable the visualization of key steps of HIV-1 item leading to nuclear import, integration into the host genome, and viral protein expression. Here, we discuss single virus imaging strategies, focusing on live-cell imaging of single virus fusion and productive uncoating that culminates in HIV-1 infection.