Combined Proteomic and In Silico Target Identification Reveal a Role for 5-Lipoxygenase in Developmental Signaling Pathways (Cell Chemical Biology)

Using cell-based screening, Brand et al. identified 3,5-substituted-2,4-dimethoxypyridines as inhibitors of Wnt signaling. Combination of chemical proteomics and computational target prediction followed by target validation revealed 5-lipoxygenase as the target.