Quantifying intracellular rates of glycolytic and oxidative ATP production and consumption using extracellular flux measurements [Additions and Corrections] (Journal of Biological Chemistry)

VOLUME 292 (2017) PAGES 7189-7207There was an error in Equation 2 on pages 7197 and 7206 and in the supporting information. The corrected equation should be ((OCRtot - OCRoli)/hyperpolarization correction factor)/?g of protein = OCRcoupled. Additionally, a hyperpolarization correction factor of 0.932 was used in some calculations instead of the value reported in the paper (0.908). Correction of these errors alters a number of the numerical values for rates of ATP synthesis in the text and in Figs. 3-6 by up to 17%, but does not affect the qualitative conclusions or any of the statements about the statistical significance. The corrected Figs. 3-6 and the supporting information have been updated with the correct values. Consequently, the following changes to the text should also be made.jbc;293/32/12649/F3F1F3Figure 3jbc;293/32/12649/F4F2F4Figure 4jbc;293/32/12649/F5F3F5Figure 5jbc;293/32/12649/F6F4F6Figure 6PAGE 7196In the second paragraph, left column, starting `JATPglyc and JATPox,` the last sentence: `The total rate of ATP production was 41.7 pmol of ATP/min/?g of cellular protein` should be: `The total rate of ATP production was 48.2 pmol of ATP/min/?g of cellular protein.`In the third paragraph, left column: `Upon the addition of glucose to provide exogenous substrate, the cells began to run glycolysis to lactate, leading to a 32% increase in the total ATP production rate to 55.2 pmol of ATP/min/?g of cellular protein. This is equivalent to about 0.2 fmol of ATP/s/cell. At the same time, oxidative ATP production decreased by 19.6% (from 38.3 to 30.8 pmol/min/?g of protein)` should be:`Upon the addition of glucose to provide exogenous substrate, the...