Project-based learning (PBL) has been widely applied to stimulate learning motivation, cultivate implementation capability, and improve learning effectiveness. The purpose of this study was to establish an assessment tool for the learning effectiveness of PBL in scientific and technological education. The methods include literature review, focus groups, and survey research. After the literature review, a draft of the scale was established, and the content validity of the scale was verified by 8 experts before being pre-tested. A total of 232 copies of valid official scales were issued and recovered. The research obtained produced subscales for “Flow experience perception”, “Self-efficacy”, “Product evaluation” and “Learning motivation”, which can be used to evaluate the learning effectiveness of the PBL in curricula of science and technology.