Spatiotemporal m(i)RNA Architecture and 3´ UTR Regulation in the C. elegans Germline (Developmental Cell)

Diag and Schilling et al. present a near single-cell gonad map of mRNAs and miRNAs and previously unannotated miRNAs and 3´ UTRs. By mutant analysis, regulators and mechanisms explaining highly dynamic spatiotemporal changes of 3´ UTR isoforms were identified. A mathematical, interrogable 3D gonad model allows comprehensive resolution of mRNA and miRNA expression in time (min) and space (~1-100 cells).