SNARE-mediated fusion of single chromaffin granules with pore-spanning membranes (Biophysical Journal)

Pore-spanning membranes (PSMs) composed of supported membrane parts (s-PSMs) as well as freestanding membrane parts (f-PSMs) are shown to be very versatile to investigate SNARE-mediated fusion on the single particle level. They provide a planar geometry readily accessible by confocal fluorescence microscopy, which enabled us for the first time to investigate the fusion of individual natural secretory granules - chromaffin granules (CGs) - on the single particle level by two color fluorescence microscopy in a time-resolved manner.