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RSS FeedsRNF12 catalyzes BRF1 ubiquitination and regulates RNA polymerase III-dependent transcription [Signal Transduction] (Journal of Biological Chemistry)

 
 

7 january 2019 09:00:21

 
RNF12 catalyzes BRF1 ubiquitination and regulates RNA polymerase III-dependent transcription [Signal Transduction] (Journal of Biological Chemistry)
 




RNA polymerase III (Pol III) is responsible for the production of small noncoding RNA species, including tRNAs and 5S rRNA. Pol III-dependent transcription is generally enhanced in transformed cells and tumors, but the underlying mechanisms remain not well-understood. It has been demonstrated that the BRF1 subunit of TFIIIB is essential for the accurate initiation of Pol III-dependent transcription. However, it is not known whether BRF1 undergoes ubiquitin modification and whether BRF1 ubiquitination regulates Pol III-dependent transcription. Here, we show that RNF12, a RING domain-containing ubiquitin E3 ligase, physically interacts with BRF1. Via direct interaction, RNF12 catalyzes Lys27- and Lys33-linked polyubiquitination of BRF1. Furthermore, RNF12 is able to negatively regulate Pol III-dependent transcription and cell proliferation via BRF1. These findings uncover a novel mechanism for the regulation of BRF1 and reveal RNF12 as an important regulator of Pol III-dependent transcription.


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32 viewsCategory: Biochemistry
 
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