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RSS FeedsIdentification of Binding Sites for Efflux Pump Inhibitors of the Escherichia coli AcrAB-TolC component AcrA (Biophysical Journal)

 
 

16 january 2019 01:00:05

 
Identification of Binding Sites for Efflux Pump Inhibitors of the Escherichia coli AcrAB-TolC component AcrA (Biophysical Journal)
 


The overexpression of multidrug efflux pumps is an important mechanism of clinical resistance in Gram-negative bacteria. Recently, four small molecules were discovered that inhibit efflux in Escherichia coli and interact with the AcrAB-TolC efflux pump component AcrA. However, the binding site(s) for these molecules was not determined. Here, we combine ensemble docking and molecular dynamics simulations with tryptophan fluorescence spectroscopy, site-directed mutagenesis, and antibiotic susceptibility assays to probe binding sites and effects of binding of these molecules.


 
74 viewsCategory: Biophysics
 
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