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RSS FeedsCasein kinase II-mediated phosphorylation of lipin 1{beta} phosphatidate phosphatase at Ser-285 and Ser-287 regulates its interaction with 14-3-3{beta} protein [Signal Transduction] (Journal of Biological Chemistry)

 
 

15 february 2019 00:01:32

 
Casein kinase II-mediated phosphorylation of lipin 1{beta} phosphatidate phosphatase at Ser-285 and Ser-287 regulates its interaction with 14-3-3{beta} protein [Signal Transduction] (Journal of Biological Chemistry)
 


The mammalian lipin 1 phosphatidate phosphatase is a key regulatory enzyme in lipid metabolism. By catalyzing phosphatidate dephosphorylation, which produces diacylglycerol, the enzyme plays a major role in the synthesis of triacylglycerol and membrane phospholipids. The importance of lipin 1 to lipid metabolism is exemplified by cellular defects and lipid-based diseases associated with its loss or overexpression. Phosphorylation of lipin 1 governs whether it is associated with the cytoplasm apart from its substrate or with the endoplasmic reticulum membrane where its enzyme reaction occurs. Lipin 1? is phosphorylated on multiple sites, but less than 10% of them are ascribed to a specific protein kinase. Here, we demonstrate that lipin 1? is a bona fide substrate for casein kinase II (CKII), a protein kinase that is essential to viability and cell cycle progression. Phosphoamino acid analysis and phosphopeptide mapping revealed that lipin 1? is phosphorylated by CKII on multiple serine and threonine residues, with the former being major sites. Mutational analysis of lipin 1? and its peptides indicated that Ser-285 and Ser-287 are both phosphorylated by CKII. Substitutions of Ser-285 and Ser-287 with nonphosphorylatable alanine attenuated the interaction of lipin 1? with 14-3-3? protein, a regulatory hub that facilitates the cytoplasmic localization of phosphorylated lipin 1. These findings advance our understanding of how phosphorylation of lipin 1? phosphatidate phosphatase regulates its interaction with 14-3-3? protein and intracellular localization and uncover a mechanism by which CKII regulates cellular physiology.


 
61 viewsCategory: Biochemistry
 
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