Vestibular dysfunction typically manifests as postural instability and gait irregularities, in part due to inaccuracies in processing spatial afference. In this study, we have instrumented the tandem walking test with multiple inertial sensors to easily and precisely investigate novel variables that can distinguish abnormal postural and gait control in patients with unilateral vestibular hypofunction. Ten healthy adults and five patients with unilateral vestibular hypofunction were assessed with the tandem walking test during eyes open and eyes closed conditions. Each subject donned five inertial sensors on the upper body (head, trunk, and pelvis) and lower body (each lateral malleolus). Our results indicate that measuring the degree of balance and gait regularity using five body-worn inertial sensors during the tandem walking test provides a novel quantification of movement that identifies abnormalities in patients with vestibular impairment.