SMALL-LABS: An algorithm for measuring single-molecule intensity and position in the presence of obscuring backgrounds (Biophysical Journal)

Single-molecule and super-resolution imaging relies on successfully, sensitively, and accurately detecting the emission from fluorescent molecules. Yet, despite the widespread adoption of super-resolution microscopies, single-molecule data processing algorithms can fail to provide accurate measurements of the brightness and position of molecules in the presence of backgrounds that fluctuate significantly over time and space. Thus, samples or experiments that include obscuring backgrounds can severely--or even completely--hinder this process.