Dynamics of the Eukaryotic Replicative Helicase at Lagging-Strand Protein Barriers Support the Steric Exclusion Model (Cell Reports)

During DNA replication, the CMG complex unwinds the double helix and must overcome protein barriers on DNA. Kose et al. show that proteins that interact exclusively with the lagging-strand template are bypassed by the helicase without stalling, implying that the lagging-strand template is completely excluded from CMG during unwinding.