Construction workers undertake demanding physical work and face high risk of injuries in poor working environments. This case-control study investigated the extent of their musculoskeletal pain incidence at work. A total of 2021 construction workers in different trades were interviewed on-site in a survey from December 2017 to December 2018. The survey results revealed that the pain prevalence of the subjects in the last 24 h was 10.6 %. The worst and top most common pain spots caused by work were central lower back, left/right shoulders, and knees. Regarding pain management, their most common method was to ignore the pain (21.4%). The average percentage of pain relief after receiving treatment in the 24 h was 37.12%. Besides, significant differences were found between the pain and non-pain groups regarding their employment duration in current job or their average sleep duration in the 24 h. The study showed that those with multiple and bilateral pain sites had pain interference on their living activities.