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RSS FeedsIJMS, Vol. 20, Pages 1920: CD20-Mimotope Peptides: A Model to Define the Molecular Basis of Epitope Spreading (International Journal of Molecular Sciences)

 
 

18 april 2019 21:01:42

 
IJMS, Vol. 20, Pages 1920: CD20-Mimotope Peptides: A Model to Define the Molecular Basis of Epitope Spreading (International Journal of Molecular Sciences)
 


Antigen-mimicking peptide (mimotope)-based vaccines are one of the most promising forms of active-immunotherapy. The main drawback of this approach is that it induces antibodies that react poorly with the nominal antigen. The aim of this study was to investigate the molecular basis underlying the weak antibody response induced against the naïve protein after peptide vaccination. For this purpose, we analyzed the fine specificity of monoclonal antibodies (mAb) elicited with a 13-mer linear peptide, complementary to theantigen-combining site of the anti-CD20 mAb, Rituximab, in BALB/c mice. Anti-peptide mAb competed with Rituximab for peptide binding. Even so, they recognized a different antigenic motif from the one recognized by Rituximab. This explains their lack of reactivity with membrane (naïve) CD20. These data indicate that even on a short peptide the immunogenic and antigenic motifs may be different. These findings highlight an additional mechanism for epitope spreading and should be taken into account when designing peptides for vaccine purposes.


 
100 viewsCategory: Biochemistry, Biophysics, Molecular Biology
 
IJMS, Vol. 20, Pages 1922: Production of Mesenchymal Stem Cells Through Stem Cell Reprogramming (International Journal of Molecular Sciences)
IJMS, Vol. 20, Pages 1921: Computational Analysis of Dengue Virus Envelope Protein (E) Reveals an Epitope with Flavivirus Immunodiagnostic Potential in Peptide Microarrays (International Journal of Molecular Sciences)
 
 
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