MyJournals Home  

RSS FeedsSpecificity from nonspecific interaction: regulation of tumor necrosis factor-{alpha} activity by DNA [Molecular Biophysics] (Journal of Biological Chemistry)

 
 

19 april 2019 14:01:32

 
Specificity from nonspecific interaction: regulation of tumor necrosis factor-{alpha} activity by DNA [Molecular Biophysics] (Journal of Biological Chemistry)
 




As anionic biopolymers, oligonucleotides can have biological functions independent from their roles as the medium for the storage and flow of genetic information. In this paper, we investigated the interaction between DNA and the pro-inflammatory cytokine tumor necrosis factor-? (TNF?). Although various forms of DNA bind to TNF? with low ?m dissociation constants, the interaction stabilizes the trimeric form of TNF? and enhances its cytotoxic effect. Based on this mechanism, a photoswitchable TNF? (TNF?-2-nitroveratryloxycarbonyl) has been designed whose sensitivity to DNA-mediated up-regulation of TNF? activity can be tuned by light irradiation. The mechanism described in this study represents a general model to understand the involvement of nonspecific interactions among biomolecules in regulating their biological functions. Because the interaction is not DNA sequence-specific, the resulting effect should be considered for oligonucleotide-based therapeutics in general.


Del.icio.us Digg Facebook Google StumbleUpon Twitter
 
15 viewsCategory: Biochemistry
 
The endoplasmic reticulum (ER) chaperones BiP and Grp94 selectively associate when BiP is in the ADP conformation [Molecular Biophysics] (Journal of Biological Chemistry)
The Legionella effector RavD binds phosphatidylinositol-3-phosphate and helps suppress endolysosomal maturation of the Legionella-containing vacuole [Membrane Biology] (Journal of Biological Chemistry)
 
 
blog comments powered by Disqus


MyJournals.org
The latest issues of all your favorite science journals on one page

Username:
Password:

Register | Retrieve

Search:

Biochemistry

Use these buttons to bookmark us:
Del.icio.us Digg Facebook Google StumbleUpon Twitter


Valid HTML 4.01 Transitional
Copyright © 2008 - 2019 Indigonet Services B.V.. Contact: Tim Hulsen. Read here our privacy notice.
Other websites of Indigonet Services B.V.: Nieuws Vacatures News Tweets Travel Photos Nachrichten Indigonet Finances Leer Mandarijn