Molecular Origin of the Stability Difference in Four Shark IgNAR Constant Domains (Biophysical Journal)

Improving stability of antibodies for manufacture and shelf life is one of the main focus of antibody engineering. One stabilization strategy is to perform specific mutations in human antibodies based on highly stable antibodies in other species. To identify the key residues for mutagenesis, it is necessary to understand the roles of these residues in stabilizing the antibody. Here, we use molecular dynamics simulations to study the molecular origin of the four shark immunoglobulin new antigen receptors (IgNAR) constant domains (C1-C4).