Live-Cell Structural Biology to Solve Biological Mechanisms: The Case of the Exocyst (Structure)

Irastorza-Azcarate et al. summarize recent cryo-EM and live-cell imaging studies on the exocyst, a protein complex involved in tethering secretory vesicles to the plasma membrane in exocytosis. The comparative analysis demonstrates the power of integrating in vitro and in situ approaches to unravel the structure and mechanism of macromolecular complexes.