MyJournals Home  

RSS FeedsToxins, Vol. 11, Pages 346: Recombinant Antibodies against Mycolactone (Toxins)


18 june 2019 00:02:37

Toxins, Vol. 11, Pages 346: Recombinant Antibodies against Mycolactone (Toxins)

In the past, it has proved challenging to generate antibodies against mycolactone, the primary lipidic toxin A of Mycobacterium ulcerans causing Buruli ulcer, due to its immunosuppressive properties. Here we show that in vitro display, comprising both phage and yeast display, can be used to select antibodies recognizing mycolactone from a large human naïve phage antibody library. Ten different antibodies were isolated, and hundreds more identified by next generation sequencing. These results indicate the value of in vitro display methods to generate antibodies against difficult antigenic targets such as toxins, which cannot be used for immunization unless inactivated by structural modification. The possibility to easily generate anti-mycolactone antibodies is an exciting prospect for the development of rapid and simple diagnostic/detection methods. Digg Facebook Google StumbleUpon Twitter
27 viewsCategory: Toxicology
Toxins, Vol. 11, Pages 347: Repertoire of the Bacillus thuringiensis Virulence Factors Unrelated to Major Classes of Protein Toxins and Its Role in Specificity of Host-Pathogen Interactions (Toxins)
Toxins, Vol. 11, Pages 345: Pharmacokinetics of Human Recombinant Anti-Botulinum Toxin Antibodies in Rats (Toxins)
blog comments powered by Disqus
The latest issues of all your favorite science journals on one page


Register | Retrieve



Use these buttons to bookmark us: Digg Facebook Google StumbleUpon Twitter

Valid HTML 4.01 Transitional
Copyright © 2008 - 2019 Indigonet Services B.V.. Contact: Tim Hulsen. Read here our privacy notice.
Other websites of Indigonet Services B.V.: Nieuws Vacatures News Tweets Travel Photos Nachrichten Indigonet Finances Leer Mandarijn