The development of Li-ion batteries has enabled the re-item of electric vehicles into the market. As car manufacturers strive to reach higher practical specific energies (550 Wh/kg) than what is achievable for Li-ion batteries, new alternatives for battery chemistry are being considered. Li-Sulfur batteries are of interest due to their ability to achieve the desired practical specific energy. The research presented in this paper focuses on the development of the Li-Sulfur technology for use in electric vehicles. The paper presents the methodology and results for endurance tests conducted on in-house manufactured Li-S cells under various accelerated ageing conditions. The Li-S cells were found to reach 80% state of health after 300–500 cycles. The results of these tests were used as the basis for discussing the second life options for Li-S batteries, as well as environmental Life Cycle Assessment results of a 50 kWh Li-S battery.