NMR structural analysis of isolated Shaker voltage-sensing domain in LPPG micelles (Biophysical Journal)

The voltage-sensing domain (VSD) is a conserved structural module that regulates the gating of voltage-dependent ion channels in response to a change in membrane potential. Although structures of many VSD containing ion channels are now available, our understanding of the structural dynamics associated with gating transitions remains limited. In order to probe dynamics with site-specific resolution, we utilized NMR spectroscopy to characterize the VSD derived from Shaker potassium channel in LPPG micelles.