MyJournals Home  

RSS FeedsIJMS, Vol. 20, Pages 3479: Development and Applications of Superfolder and Split Fluorescent Protein Detection Systems in Biology (International Journal of Molecular Sciences)

 
 

15 july 2019 20:00:04

 
IJMS, Vol. 20, Pages 3479: Development and Applications of Superfolder and Split Fluorescent Protein Detection Systems in Biology (International Journal of Molecular Sciences)
 


Molecular engineering of the green fluorescent protein (GFP) into a robust and stable variant named Superfolder GFP (sfGFP) has revolutionized the field of biosensor development and the use of fluorescent markers in diverse area of biology. sfGFP-based self-associating bipartite split-FP systems have been widely exploited to monitor soluble expression in vitro, localization, and trafficking of proteins in cellulo. A more recent class of split-FP variants, named « tripartite » split-FP, that rely on the self-assembly of three GFP fragments, is particularly well suited for the detection of protein–protein interactions. In this review, we describe the different steps and evolutions that have led to the diversification of superfolder and split-FP reporter systems, and we report an update of their applications in various areas of biology, from structural biology to cell biology.


 
223 viewsCategory: Biochemistry, Biophysics, Molecular Biology
 
IJMS, Vol. 20, Pages 3480: Discovering and Constructing ceRNA-miRNA-Target Gene Regulatory Networks during Anther Development in Maize (International Journal of Molecular Sciences)
IJMS, Vol. 20, Pages 3478: Global DNA Methylation Patterns in Human Gliomas and Their Interplay with Other Epigenetic Modifications (International Journal of Molecular Sciences)
 
 
blog comments powered by Disqus


MyJournals.org
The latest issues of all your favorite science journals on one page

Username:
Password:

Register | Retrieve

Search:

Molecular Biology


Copyright © 2008 - 2024 Indigonet Services B.V.. Contact: Tim Hulsen. Read here our privacy notice.
Other websites of Indigonet Services B.V.: Nieuws Vacatures News Tweets Nachrichten