The article deals with the development of a mechatronic system for locking vehicle differentials. An important benefit of this system is that it prevents the jamming of the vehicle in difficult adhesion conditions. The system recognizes such a situation much sooner than the driver and is able to respond immediately, ensuring smooth driving in off-road or snowy conditions. This article describes the control algorithm of this mechatronic system, which is designed for firefighting, military, or civilian vehicles with a drivetrain configuration of up to 10 × 10, and also explains the input signal processing and the control of actuators. The main part of this article concerns prototype testing on a vehicle. The results are an evaluation of one of the many experiments and monitor the proper function of the developed mechatronic system.