Customizing Functionalized Cofactor Mimics to Study the Human Pyridoxal 5´-Phosphate-Binding Proteome (Cell Chemical Biology)

Fux et al. applied cofactor mimics to study human pyridoxal 5´-phosphate (PLP)-dependent proteins in situ, covering a large fraction of known vitamin B6-binders. Moreover, cellular targets of anti-vitamin B6 compounds were unraveled. Comparison of different human cell lines resulted in signature labeling profiles, suggesting different cofactor loading states of PLP-binding proteins.