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RSS FeedsIJMS, Vol. 20, Pages 4558: Insight into the Antifungal Mechanism of Action of Human RNase N-terminus Derived Peptides (International Journal of Molecular Sciences)

 
 

14 september 2019 10:00:41

 
IJMS, Vol. 20, Pages 4558: Insight into the Antifungal Mechanism of Action of Human RNase N-terminus Derived Peptides (International Journal of Molecular Sciences)
 


Candida albicans is a polymorphic fungus responsible for mucosal and skin infections. Candida cells establish themselves into biofilm communities resistant to most currently available antifungal agents. An increase of severe infections ensuing in fungal septic shock in elderly or immunosuppressed patients, along with the emergence of drug-resistant strains, urge the need for the development of alternative antifungal agents. In the search for novel antifungal drugs our laboratory demonstrated that two human ribonucleases from the vertebrate-specific RNaseA superfamily, hRNase3 and hRNase7, display a high anticandidal activity. In a previous work, we proved that the N-terminal region of the RNases was sufficient to reproduce most of the parental protein bactericidal activity. Next, we explored their potency against a fungal pathogen. Here, we have tested the N-terminal derived peptides that correspond to the eight human canonical RNases (RN1-8) against planktonic cells and biofilms of C. albicans. RN3 and RN7 peptides displayed the most potent inhibitory effect with a mechanism of action characterized by cell-wall binding, membrane permeabilization and biofilm eradication activities. Both peptides are able to eradicate planktonic and sessile cells, and to alter their gene expression, reinforcing its role as a lead candidate to develop novel antifungal and antibiofilm therapies.


 
208 viewsCategory: Biochemistry, Biophysics, Molecular Biology
 
IJMS, Vol. 20, Pages 4552: Enhanced Function and Overexpression of Metabotropic Glutamate Receptors 1 and 5 in the Spinal Cord of the SOD1G93A Mouse Model of Amyotrophic Lateral Sclerosis during Disease Progression (International Journal of Molecular Sciences)
IJMS, Vol. 20, Pages 4557: Deletion of a Seminal Gene Cluster Reinforces a Crucial Role of SVS2 in Male Fertility (International Journal of Molecular Sciences)
 
 
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