MyJournals Home  

RSS FeedsChromatin-sensitive cryptic promoters putatively drive expression of alternative protein isoforms in yeast [RESEARCH] (Genome Research)

 
 

2 december 2019 21:03:01

 
Chromatin-sensitive cryptic promoters putatively drive expression of alternative protein isoforms in yeast [RESEARCH] (Genome Research)
 




Cryptic transcription is widespread and generates a heterogeneous group of RNA molecules of unknown function. To improve our understanding of cryptic transcription, we investigated their transcription start site (TSS) usage, chromatin organization, and posttranscriptional consequences in Saccharomyces cerevisiae. We show that TSSs of chromatin-sensitive internal cryptic transcripts retain comparable features of canonical TSSs in terms of DNA sequence, directionality, and chromatin accessibility. We define the 5` and 3` boundaries of cryptic transcripts and show that, contrary to RNA degradation–sensitive ones, they often overlap with the end of the gene, thereby using the canonical polyadenylation site, and associate to polyribosomes. We show that chromatin-sensitive cryptic transcripts can be recognized by ribosomes and may produce truncated polypeptides from downstream, in-frame start codons. Finally, we confirm the presence of the predicted polypeptides by reanalyzing N-terminal proteomic data sets. Our work suggests that a fraction of chromatin-sensitive internal cryptic promoters initiates the transcription of alternative truncated mRNA isoforms. The expression of these chromatin-sensitive isoforms is conserved from yeast to human, expanding the functional consequences of cryptic transcription and proteome complexity.


Del.icio.us Digg Facebook Google StumbleUpon Twitter
 
35 viewsCategory: Bioinformatics, Genetics, Genomics
 
A high-resolution gene expression atlas links dedicated meristem genes to key architectural traits [RESEARCH] (Genome Research)
Accessibility of promoter DNA is not the primary determinant of chromatin-mediated gene regulation [RESEARCH] (Genome Research)
 
 
blog comments powered by Disqus


MyJournals.org
The latest issues of all your favorite science journals on one page

Username:
Password:

Register | Retrieve

Search:

Genomics

Use these buttons to bookmark us:
Del.icio.us Digg Facebook Google StumbleUpon Twitter


Valid HTML 4.01 Transitional
Copyright © 2008 - 2019 Indigonet Services B.V.. Contact: Tim Hulsen. Read here our privacy notice.
Other websites of Indigonet Services B.V.: Nieuws Vacatures News Tweets Travel Photos Nachrichten Indigonet Finances Leer Mandarijn