Ni-free orthodontic wires were introduced to mitigate concerns associated with the use of Ni-containing alloys in orthodontics. However, limited information is available on their properties and therefore, the aim of this study was to characterize the elemental composition, the microstructure, and the mechanical properties of Ni-free orthodontic wires and compare them with their stainless steel (SS) counterparts. Four Ni-free and four conventional SS wires were included in this study. All the wires were initially imaged with a Scanning Electron Microscopy (SEM) and their elemental compositions were determined by X-ray Energy Dispersive Spectroscopy (EDX). Then, their microstructure was assessed by X-ray Diffraction (XRD) and the indentation modulus, elastic index, Martens Hardness and Vickers Hardness by Instrumented Indentation Testing (IIT). All the wires demonstrated surface cracks and pores oriented parallel to their long axis. The elemental composition of Ni-free alloys showed an increased Mn and Cr content while both SS and Ni-free wires shared the same dominant austenite structure. In conclusion, despite the differences in elemental composition, Ni-free wires demonstrated a similar microstructure and comparable mechanical properties with their conventional SS counterparts and thus may be considered as a promising alternative for patients with Ni supersensitivity.