Duplex stainless steels (DSSs), a particular category of stainless steels, are employed in all kinds of industrial applications where excellent corrosion resistance and high strength are necessary. These good properties are provided by their biphasic microstructure, consisting of ferrite and austenite in almost equal volume fractions of phases. In the present work, Nd: YAG pulsed laser dissimilar welding of UNS S32750 super duplex stainless steel (SDSS) with 316L austenitic stainless steel (ASS), with different heat inputs, was investigated. The results showed that the fusion zone microstructure observed consisted of a ferrite matrix with grain boundary austenite (GBA), Widmanstätten austenite (WA) and intragranular austenite (IA), with the same proportion of ferrite and austenite phases. Changes in the heat input (between 45, 90 and 120 J/mm) did not significantly affect the ferrite/austenite phase balance and the microhardness in the fusion zone.