Quantitative Multiplexed ChIP Reveals Global Alterations that Shape Promoter Bivalency in Ground State Embryonic Stem Cells (Cell Reports)

Kumar et al. employ an improved multiplexed quantitative ChIP-seq methodology to characterize mESC pluripotency. They reveal that bivalent promoters are retained in the 2i ground state, which is characterized by abundant H3K27me3. H3K4me3 levels are high in serum-primed state and a stable 2i/serum intermediate but strikingly reduced in ground state.