MyJournals Home  

RSS FeedsMolecules, Vol. 25, Pages 353: High-Throughput Screening Identifies Two Novel Small Molecule Enhancers of Recombinant Protein Expression (Molecules)

 
 

15 january 2020 21:02:39

 
Molecules, Vol. 25, Pages 353: High-Throughput Screening Identifies Two Novel Small Molecule Enhancers of Recombinant Protein Expression (Molecules)
 


As a primary strategy for production of biological drugs, recombinant proteins produced by transient transfection of mammalian cells are essential for both basic research and industrial production. Here, we established a high-throughput screening platform for improving the expression levels of recombinant proteins. In total, 10,011 small molecule compounds were screened through our platform. After two rounds of screening, we identified two compounds, Apicidin and M-344, that significantly enhanced recombinant protein expression. Both of the selected compounds were histone deacetylase inhibitors, suggesting that the two small molecules increased the expression levels of recombinant proteins by promoting histone acetylation. Moreover, both molecules showed low cytotoxicity. Therefore, our findings suggest that these small molecules may have wide applications in the future.


 
137 viewsCategory: Biochemistry, Chemistry, Molecular Biology
 
Molecules, Vol. 25, Pages 354: The Effects of Ultraviolet A/B Treatments on Anthocyanin Accumulation and Gene Expression in Dark-Purple Tea Cultivar `Ziyan` (Camellia sinensis) (Molecules)
Molecules, Vol. 25, Pages 352: Tunable Aryl Imidazolium Recyclable Ionic Liquid with Dual Brønsted-Lewis Acid as Green Catalyst for Friedel-Crafts Acylation and Thioesterification (Molecules)
 
 
blog comments powered by Disqus


MyJournals.org
The latest issues of all your favorite science journals on one page

Username:
Password:

Register | Retrieve

Search:

Molecular Biology


Copyright © 2008 - 2020 Indigonet Services B.V.. Contact: Tim Hulsen. Read here our privacy notice.
Other websites of Indigonet Services B.V.: Nieuws Vacatures News Tweets Nachrichten