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RSS FeedsSMARCAD1-mediated recruitment of the DNA mismatch repair protein MutL{alpha} to MutS{alpha} on damaged chromatin induces apoptosis in human cells [DNA and Chromosomes] (Journal of Biological Chemistry)

 
 

24 january 2020 10:01:19

 
SMARCAD1-mediated recruitment of the DNA mismatch repair protein MutL{alpha} to MutS{alpha} on damaged chromatin induces apoptosis in human cells [DNA and Chromosomes] (Journal of Biological Chemistry)
 


The mismatch repair (MMR) complex is composed of MutS? (MSH2-MSH6) and MutL? (MLH1-PMS2) and specifically recognizes mismatched bases during DNA replication. O6-Methylguanine is produced by treatment with alkylating agents, such as N-methyl-N-nitrosourea (MNU), and during DNA replication forms a DNA mismatch (i.e. an O6-methylguanine/thymine pair) and induces a G/C to A/T transition mutation. To prevent this outcome, cells carrying this DNA mismatch are eliminated by MMR-dependent apoptosis, but the underlying molecular mechanism is unclear. In this study, we provide evidence that the chromatin-regulatory and ATP-dependent nucleosome-remodeling protein SMARCAD1 is involved in the induction of MMR-dependent apoptosis in human cells. Unlike control cells, SMARCAD1-knockout cells (?SMARCAD1) were MNU-resistant, and the appearance of a sub-G1 population and caspase-9 activation were significantly suppressed in the ?SMARCAD1 cells. Furthermore, the MNU-induced mutation frequencies were increased in these cells. Immunoprecipitation analyses revealed that the recruitment of MutL? to chromatin-bound MutS?, observed in SMARCAD1-proficient cells, is suppressed in ?SMARCAD1 cells. Of note, the effect of SMARCAD1 on the recruitment of MutL? exclusively depended on the ATPase activity of the protein. On the basis of these findings, we propose that SMARCAD1 induces apoptosis via its chromatin-remodeling activity, which helps recruit MutL? to MutS? on damaged chromatin.


 
289 viewsCategory: Biochemistry
 
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