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RSS FeedsIJMS, Vol. 21, Pages 2290: Upregulation of CASP9 through NF-?B and Its Target MiR-1276 Contributed to TNF?-promoted Apoptosis of Cancer Cells Induced by Doxorubicin (International Journal of Molecular Sciences)

 
 

26 march 2020 21:00:46

 
IJMS, Vol. 21, Pages 2290: Upregulation of CASP9 through NF-?B and Its Target MiR-1276 Contributed to TNF?-promoted Apoptosis of Cancer Cells Induced by Doxorubicin (International Journal of Molecular Sciences)
 


:Under some conditions, nuclear factor-κB (NF-κB) has a pro-apoptotic role, but the mechanisms underlying this function remain unclear. This study demonstrated that NF-κB directly binds to CASP9 and miR1276 in tumor necrosis factor α (TNFα)-treated HeLa and HepG2 cells. NF-κB upregulated CASP9 expression, whereas downregulated miR1276 expression in the TNFα-treated cells. The miR1276 repressed CASP9 expression in both cells. As a result, a typical NF-κB-mediated coherent feed-forward loop was formed in the TNFα-treated cells. It was proposed that the NF-κB-mediated loop may contribute to cell apoptosis under certain conditions. This opinion was supported by the following evidence: TNFα promoted the apoptosis of HeLa and HepG2 cells induced by doxorubicin (DOX). CASP9 was significantly upregulated and activated by TNFα in the DOX-induced cells. Moreover, a known inhibitor of CASP9 activation significantly repressed the TNFα promotion of apoptosis induced by DOX. These findings indicate that CASP9 is a new mediator of the NF-κB pro-apoptotic pathway, at least in such conditions. This study therefore provides new insights into the pro-apoptotic role of NF-κB. The results also shed new light on the molecular mechanism underlying TNFα-promotion of cancer cells apoptosis induced by some anticancer drugs such as DOX.


 
199 viewsCategory: Biochemistry, Biophysics, Molecular Biology
 
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IJMS, Vol. 21, Pages 2291: Cold Atmospheric Plasma Treatment of Chondrosarcoma Cells Affects Proliferation and Cell Membrane Permeability (International Journal of Molecular Sciences)
 
 
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