In Vitro and In Vivo Development of the Human Airway at Single-Cell Resolution (Developmental Cell)

Miller et al. show that transient SMAD activation followed by inhibition patterns human lung bud tip progenitor organoids to functional airway cell types, including basal cells. Comparison of the organoids and the human lung epithelium developing in vivo by scRNA-seq demonstrates a high degree of transcriptomic similarity.