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RSS FeedsIJMS, Vol. 22, Pages 12821: Root-to-Shoot Long-Distance Mobile miRNAs Identified from Nicotiana Rootstocks (International Journal of Molecular Sciences)

 
 

26 november 2021 16:28:45

 
IJMS, Vol. 22, Pages 12821: Root-to-Shoot Long-Distance Mobile miRNAs Identified from Nicotiana Rootstocks (International Journal of Molecular Sciences)
 


Root-derived mobile signals play critical roles in coordinating a shoot’s response to underground conditions. However, the identification of root-to-shoot long-distance mobile signals has been scant. In this study, we aimed to characterize root-to-shoot endogenous mobile miRNAs by using an Arabidopsis/Nicotiana interfamilial heterograft in which these two taxonomically distant species with clear genetic backgrounds had sufficient diversity in differentiating miRNA sources. Small RNA deep sequencing analysis revealed that 82 miRNAs from the Arabidopsis scion could travel through the graft union to reach the rootstock, whereas only a very small subset of miRNA (6 miRNAs) preferred the root-to-shoot movement. We demonstrated in an ex vivo RNA imaging experiment that the root-to-shoot mobile Nb-miR164, Nb-miR395 and Nb-miR397 were targeted to plasmodesmata using the bacteriophage coat protein MS2 system. Furthermore, the Nb-miR164 was shown to move from the roots to the shoots to induce phenotypic changes when its overexpressing line was used as rootstock, strongly supporting that root-derived Nb-miR164 was able to modify the scion trait via its long-distance movement.


 
128 viewsCategory: Biochemistry, Biophysics, Molecular Biology
 
IJMS, Vol. 22, Pages 12824: The Flavonoid Biosynthesis Network in Plants (International Journal of Molecular Sciences)
IJMS, Vol. 22, Pages 12822: Molecular Characterization of Four Alkaline Chitinases from Three Chitinolytic Bacteria Isolated from a Mudflat (International Journal of Molecular Sciences)
 
 
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