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RSS FeedsIJMS, Vol. 23, Pages 1335: Fabrication of Fragment Antibody–Enzyme Complex as a Sensing Element for Immunosensing (International Journal of Molecular Sciences)

 
 

25 january 2022 10:58:11

 
IJMS, Vol. 23, Pages 1335: Fabrication of Fragment Antibody–Enzyme Complex as a Sensing Element for Immunosensing (International Journal of Molecular Sciences)
 


Antibody–enzyme complexes (AECs) are ideal molecular recognition elements for immunosensing applications. One molecule possesses both a binding ability to specific targets and catalytic activity to gain signals, particularly oxidoreductases, which can be integrated into rapid and sensitive electrochemical measurements. The development of AECs using fragment antibodies rather than intact antibodies, such as immunoglobulin G (IgG), has attracted attention for overcoming the ethical and cost issues associated with the production of intact antibodies. Conventionally, chemical conjugation has been used to fabricate AECs; however, controlling stoichiometric conjugation using this method is difficult. To prepare homogeneous AECs, methods based on direct fusion and enzymatic conjugation have been developed, and more convenient methods using Catcher/Tag systems as coupling modules have been reported. In this review, we summarize the methods for fabricating AECs using fragment antibodies developed for sensing applications and discuss the advantages and disadvantages of each method.


 
62 viewsCategory: Biochemistry, Biophysics, Molecular Biology
 
IJMS, Vol. 23, Pages 1334: Expression of Doublecortin, Glial Fibrillar Acidic Protein, and Vimentin in the Intact Subpallium and after Traumatic Injury to the Pallium in Juvenile Salmon, Oncorhynchus masou (International Journal of Molecular Sciences)
IJMS, Vol. 23, Pages 1336: Determination of the Optimal Bacterial DNA Extraction Method to Explore the Urinary Microbiota (International Journal of Molecular Sciences)
 
 
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