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RSS FeedsIJMS, Vol. 23, Pages 5808: Dynamic Changes in Ascorbic Acid Content during Fruit Development and Ripening of Actinidia latifolia (An Ascorbate-Rich Fruit Crop) and the Associated Molecular Mechanisms (International Journal of Molecular Sciences)

 
 

22 may 2022 11:20:40

 
IJMS, Vol. 23, Pages 5808: Dynamic Changes in Ascorbic Acid Content during Fruit Development and Ripening of Actinidia latifolia (An Ascorbate-Rich Fruit Crop) and the Associated Molecular Mechanisms (International Journal of Molecular Sciences)
 


Actinidia latifolia is one of the very few kiwifruit genotypes with extremely high ascorbic acid (AsA) content. However, a transcriptome atlas of this species is lacking. The accumulation of AsA during fruit development and ripening and the associated molecular mechanisms are still poorly understood. Herein, dynamic changes in AsA content at six different stages of A. latifolia fruit development and ripening were determined. AsA content of A. latifolia fruit reached 1108.76 ± 35.26 mg 100 g−1 FW at full maturity. A high-quality, full-length (FL) transcriptome of A. latifolia was successfully constructed for the first time using third-generation sequencing technology. The transcriptome comprises 326,926 FL non-chimeric reads, 15,505 coding sequences, 2882 transcription factors, 18,797 simple sequence repeats, 3328 long noncoding RNAs, and 231 alternative splicing events. The genes involved in AsA biosynthesis and recycling pathways were identified and compared with those in different kiwifruit genotypes. The correlation between the AsA content and expression levels of key genes in AsA biosynthesis and recycling pathways was revealed. LncRNAs that participate in AsA-related gene expression regulation were also identified. Gene expression patterns in AsA biosynthesis and metabolism exhibited a trend similar to that of AsA accumulation. Overall, this study paves the way for genetic engineering to develop kiwifruits with super-high AsA content.


 
71 viewsCategory: Biochemistry, Biophysics, Molecular Biology
 
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