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RSS FeedsIJMS, Vol. 23, Pages 11365: Synthetic Non−Coding RNA for Suppressing mTOR Translation to Prevent Renal Fibrosis Related to Autophagy in UUO Mouse Model (International Journal of Molecular Sciences)

 
 

26 september 2022 16:04:48

 
IJMS, Vol. 23, Pages 11365: Synthetic Non−Coding RNA for Suppressing mTOR Translation to Prevent Renal Fibrosis Related to Autophagy in UUO Mouse Model (International Journal of Molecular Sciences)
 


The global burden of chronic kidney disease is increasing, and the majority of these diseases are progressive. Special site−targeted drugs are emerging as alternatives to traditional drugs. Oligonucleotides (ODNs) have been proposed as effective therapeutic tools in specific molecular target therapies for several diseases. We designed ring−type non−coding RNAs (ncRNAs), also called mTOR ODNs to suppress mammalian target rapamycin (mTOR) translation. mTOR signaling is associated with excessive cell proliferation and fibrogenesis. In this study, we examined the effects of mTOR suppression on chronic renal injury. To explore the regulation of fibrosis and inflammation in unilateral ureteral obstruction (UUO)−induced injury, we injected synthesized ODNs via the tail vein of mice. The expression of inflammatory−related markers (interleukin−1β, tumor necrosis factor−α), and that of fibrosis (α−smooth muscle actin, fibronectin), was decreased by synthetic ODNs. Additionally, ODN administration inhibited the expression of autophagy−related markers, microtubule−associated protein light chain 3, Beclin1, and autophagy−related gene 5−12. We confirmed that ring−type ODNs inhibited fibrosis, inflammation, and autophagy in a UUO mouse model. These results suggest that mTOR may be involved in the regulation of autophagy and fibrosis and that regulating mTOR signaling may be a therapeutic strategy against chronic renal injury.


 
91 viewsCategory: Biochemistry, Biophysics, Molecular Biology
 
IJMS, Vol. 23, Pages 11362: Evaluating the Safety and Quality of Life of Colorectal Cancer Patients Treated by Autologous Immune Enhancement Therapy (AIET) in Vinmec International Hospitals (International Journal of Molecular Sciences)
IJMS, Vol. 23, Pages 11364: Theoretical Explanation for the Rarity of Antibody-Dependent Enhancement of Infection (ADE) in COVID-19 (International Journal of Molecular Sciences)
 
 
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