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RSS FeedsIJERPH, Vol. 19, Pages 12488: Quantification of Plasma 8-Isoprostane by High-Performance Liquid Chromatography with Tandem Mass Spectrometry in a Case-Control Study of Lung Cancer (International Journal of Environmental Research and Public Health)

 
 

30 september 2022 17:52:19

 
IJERPH, Vol. 19, Pages 12488: Quantification of Plasma 8-Isoprostane by High-Performance Liquid Chromatography with Tandem Mass Spectrometry in a Case-Control Study of Lung Cancer (International Journal of Environmental Research and Public Health)
 


Aim: 8-iso-prostaglandin F2α is a biomarker of lipid peroxidation, and one of the most commonly used measures of oxidative stress. It is an established biomarker of lung cancer risk. It is commonly measured by enzyme-linked immunosorbent assay. Given its importance, we developed a stable isotope dilution UPLC-tandem mass spectrometric method for the rapid determination of 8-isoprostane in blood. Methods: We tested the discriminatory capability of the method in 49 lung cancer patients, 55 benign lung nodule patients detected by chest X-ray, and 41 patients with chronic obstructive pulmonary disease (COPD) or asthma. Results: Significant differences were found in mean 8-isoprostane levels between the three groups (p = 0.027), and post-hoc tests found higher levels in the lung cancer patients than in patients with benign nodules (p = 0.032) and COPD/asthma (p = 0.014). The receiving operating characteristic area under the curve (AUC) was 0.69 for differentiating the lung cancer group from the benign nodule group, and 0.7 for differentiating from the COPD/asthma group. Conclusions: The UPLC-MS/MS-based method is an efficient analytical tool for measuring 8-isoprostane plasma concentrations. The results suggest exploring its utility as a marker for early lung cancer screening.


 
84 viewsCategory: Medicine, Pathology, Toxicology
 
IJERPH, Vol. 19, Pages 12526: Impact of Sociodemographic Factors on Use of Formal Social Services in an Older Swedish Population (International Journal of Environmental Research and Public Health)
IJERPH, Vol. 19, Pages 12527: Assessing Smoking Habits, Attitudes, Knowledge, and Needs among University Students at the University of Milan, Italy (International Journal of Environmental Research and Public Health)
 
 
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