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RSS FeedsMarine Drugs, Vol. 20, Pages 741: κ- and λ-Carrageenans from Marine Alga Сhondrus armatus Exhibit Anticancer In Vitro Activity in Human Gastrointestinal Cancers Models (Marine Drugs)

 
 

25 november 2022 16:25:46

 
Marine Drugs, Vol. 20, Pages 741: κ- and λ-Carrageenans from Marine Alga Сhondrus armatus Exhibit Anticancer In Vitro Activity in Human Gastrointestinal Cancers Models (Marine Drugs)
 


The carrageenans isolated from red algae demonstrated a variety of activities from antiviral and immunomodulatory to antitumor. The diverse structure and sulfation profile of carrageenans provide a great landscape for drug development. In this study, we isolated, purified and structurally characterized κо- and λо- oligosaccharides from the marine algae Chondrus armatus. We further examined the tumor suppressive activity of both carrageenans in gastrointestinal cancer models. Thus, using MTT assay, we could demonstrate a pronounced antiproliferative effect of the carrageenans in KYSE-30 and FLO-1 as well as HCT-116 and RKO cell lines with IC50 184~405 μg/mL, while both compounds were less active in non-cancer epithelial cells RPE-1. This effect was stipulated by the inhibition of cell cycle progression in the cancer cells. Specifically, flow cytometry revealed an S phase delay in FLO-1 and HCT-116 cells under κo-carrageenan treatment, while KYSE-30 demonstrated a pronounced G2/M cell cycle delay. In line with this, western blotting revealed a reduction of cell cycle markers CDK2 and E2F2. Interestingly, κo-carrageenan inhibited cell cycle progression of RKO cells in G1 phase. Finally, isolated κo- and λo- carrageenans induced apoptosis on adenocarcinomas, specifically with high apoptosis induction in RKO cells. Overall, our data underline the potential of κo- and λo- carrageenans for colon and esophageal carcinoma drug development.


 
83 viewsCategory: Biochemistry, Molecular Biology, Pharmacology
 
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