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RSS FeedsIJMS, Vol. 23, Pages 14889: Calix[4]arene Polyamine Triazoles: Synthesis, Aggregation and DNA Binding (International Journal of Molecular Sciences)

 
 

28 november 2022 13:13:21

 
IJMS, Vol. 23, Pages 14889: Calix[4]arene Polyamine Triazoles: Synthesis, Aggregation and DNA Binding (International Journal of Molecular Sciences)
 


Artificial gene delivery systems are in great demand from both scientific and practical biomedical points of view. In this paper, we present the synthesis of a new click chemistry calix[4]arene precursor with free lower rim and new water-soluble calixarene triazoles with 12 amino-groups on the upper rim (one with free phenol hydroxyl groups and two another containing four butyl or tetradecyl fragments). Aggregation in the series of amino-triazole calixarenes of different lipophilicity (calixarene with free phenol hydroxyl groups or butyl and tetradecyl fragments on the lower rim) was studied using dynamic light scattering and fluorescent pyrene probe. It was found that calix[4]arene with a free lower rim, like alkyl-substituted butyl calix[4]arene, forms stable submicron aggregates 150–200 nm in size, while the more lipophilic tetradecyl –substituted calix[4]arene forms micellar aggregates19 nm in size. Using UV-Vis spectroscopy, fluorimetry and CD, it was shown that amino-triazole calix[4]arenes bind to calf thymus DNA by classical intercalation. According to DLS and TEM data, all studied macrocycles cause significant DNA compaction, forming stable nanoparticles 50–20 nm in size. Among all studied calix[4]arenes the most lipophilic tetradecyl one proved to be the best for both binding and compaction of DNA.


 
83 viewsCategory: Biochemistry, Biophysics, Molecular Biology
 
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