The relationship between deiodinase activity and inflammatory responses under the stimulation of uremic toxins (Journal of Translational Medicine)

Background: It is unclear to what extent uremic toxins participate in inflammatory responses and the activities of deiodinases, as well as the effects of deiodinases on inflammatory cytokines.Materials and methodsHepatocellular carcinoma cell lines (HepG2) were transfected with small interfering ribonucleic acid (siRNA) specific for deiodinase type 1 (DIO1) and cultured with or without uremic toxins. The mRNA expression of DIO1, interleukin (IL)-1?, IL-6, and tumor necrosis factor (TNF)-? was detected by quantitative real-time PCR. The presence of selenoprotein M (SelM) and DIO1 was assessed by western blotting. Sonicate deiodinase activities in HepG2 cells were measured by a dithiothreitol-stimulated assay. The NF-?B, AP-1 and CREB-1 inflammatory signal pathways were confirmed by EMSA. Results: After culturing for 24?h, the mRNA expression of DIO1 was significantly decreased by the specific siRNA (reduced by 76%, P?=?0.0002). Uremic toxins significantly increased the mRNA expression (P?