A Chemical Proteomics Approach to Reveal Direct Protein-Protein Interactions in Living Cells (Cell Chemical Biology)

Protein-protein interactions mediate essential biological processes, but characterizing these interactions in cells presents a major challenge. Kleiner et al. describe a chemical proteomics approach combining photo-crosslinking and SILAC-based proteomics to characterize context-dependent direct protein-protein interactions in live cells. They apply their approach to profile interactions involving core histones H3 and H4.