Global warming caused by human activities exacerbates the water cycle, changes precipitation features, such as precipitation amount, intensity and time, and raises uncertainties in water resources. This work uses run-off data obtained using climate change models under representative concentration pathways (RCPs) and selects the Yangtze River Basin as the research boundary to evaluate and analyse the vulnerability of hydropower generation in 2016-2050 on the basis of the water evaluation and planning model. Results show that the amount of rainfall during 2016-2050 in the Yangtze River Basin is estimated to increase with fluctuations in RCP4.5 and RCP8.5 scenarios. In the RCP4.5 scenario, hydropower stations exhibit large fluctuations in generating capacity, which present the trend of an increase after a decrease; in the RCP8.5 scenario, the generating capacity of hydropower stations in the Yangtze River Basin presents a steady increase. Over 50% of the generating capacity in the Yangtze River Basin is produced from the Three Gorges Dam and 10 other hydropower stations. Over 90% is generated in eight river basins, including the Jinsha, Ya-lung and Min Rivers. Therefore, climate change may accelerate changes in the Yangtze River Basin and further lead to vulnerability of hydropower generation.