MyJournals Home  

RSS FeedsMarine Drugs, Vol. 16, Pages 15: Aquaculture Soft Coral Lobophytum crassum as a Producer of Anti-Proliferative Cembranoids (Marine Drugs)

 
 

7 january 2018 13:15:08

 
Marine Drugs, Vol. 16, Pages 15: Aquaculture Soft Coral Lobophytum crassum as a Producer of Anti-Proliferative Cembranoids (Marine Drugs)
 




Our continuous search for marine bioactive secondary metabolites led to the screening of crude extracts from a variety of aquaculture soft corals. The ethyl acetate (EtOAc) extract of Lobophytum crassum showed a distinctive chemical profile that was different from the wild type. It demonstrated significant anti-proliferative activity against Molt 4 leukemia cell with an IC50 value of 1 ?g/mL after 24 h. Chemical investigation focusing on the unique peaks in L. crassum profile led to the discovery of a new ?-tocopherol crassumtocopherol C (1), and two new cembrane-based diterpenoids culobophylins D (2) and E (3), along with ten known cembranoids (4-13). The structures of these isolates were elucidated using extensive spectroscopic techniques and a comparison with previously published data of related metabolites. Compound 2 was found to possess the first identified saturated internal C4-O-C14 linkage six-membered ring among all cembrane-type diterpenoids. The anti-proliferative activity of all the isolates (except 3) was evaluated against a limited panel of leukemia cell lines (Molt 4, K562, U937, and Sup-T1). The major compounds 8 and 10 exhibited the most anti-proliferative potent effect, with IC50 values ranging from 1.2 to 7.1 ?M. The Structure Activity Relationship (SAR) of the isolates suggested that the presence of lactone moieties is crucial for the anti-proliferative activity against leukemia cells. Our work indicated that the development of an efficient aquaculture protocols for soft corals led to the discovery of new secondary metabolites with unique structural features. Such protocols can lead to a sustainable supply of biologically active compounds in enough quantities for the pharmaceutical industry.


Del.icio.us Digg Facebook Google StumbleUpon Twitter
 
76 viewsCategory: Biochemistry, Molecular Biology, Pharmacology
 
Marine Drugs, Vol. 16, Pages 12: Secondary Metabolites of Mangrove-Associated Strains of Talaromyces (Marine Drugs)
Marine Drugs, Vol. 16, Pages 30: Characterization of a Novel Alginate Lyase from Marine Bacterium Vibrio furnissii H1 (Marine Drugs)
 
 
blog comments powered by Disqus


MyJournals.org
The latest issues of all your favorite science journals on one page

Username:
Password:

Register | Retrieve

Search:

Pharmacology

Use these buttons to bookmark us:
Del.icio.us Digg Facebook Google StumbleUpon Twitter


Valid HTML 4.01 Transitional
Copyright © 2008 - 2018 Indigonet Services B.V.. Contact: Tim Hulsen
Other websites of Indigonet Services B.V.: Nieuws Vacatures News Tweets Travel Photos Nachrichten Indigonet Finances Leer Mandarijn
Indigonet Services BV is not responsible for any incorrect or outdated information on this site.
If, however, you would like to see an article removed, you can send us an e-mail quoting the article number.