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RSS FeedsToxins, Vol. 10, Pages 195: Purification and Characterization of Recombinant Botulinum Neurotoxin Serotype FA, Also Known as Serotype H (Toxins)

 
 

16 may 2018 00:00:20

 
Toxins, Vol. 10, Pages 195: Purification and Characterization of Recombinant Botulinum Neurotoxin Serotype FA, Also Known as Serotype H (Toxins)
 




We have purified and characterized recombinant botulinum neurotoxin serotype FA (BoNT/FA). This protein has also been named as a new serotype (serotype H), but the classification has been controversial. A lack of well-characterized, highly pure material has been a roadblock to study. Here we report purification and characterization of enzymatically active, and of inactive nontoxic, recombinant forms of BoNT/FA as tractable alternatives to purifying this neurotoxin from native Clostridium botulinum. BoNT/FA cleaves the same intracellular target proteins as BoNT/F1 and other F serotype BoNTs; the intracellular targets are vesicle associated membrane proteins (VAMP) 1, 2 and 3. BoNT/FA cleaves the same site in VAMP-2 as BoNT/F5, which is different from the cleavage site of other F serotype BoNTs. BoNT/FA has slower enzyme kinetics than BoNT/F1 in a cell-free protease assay and is less potent at inhibiting ex vivo nerve-stimulated skeletal muscle contraction. In contrast, BoNT/FA is more potent at inhibiting neurotransmitter release from cultured neurons.


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86 viewsCategory: Toxicology
 
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