Hydrophobic mismatch modulates stability and plasticity of human mitochondrial VDAC2 (Biophysical Journal)

The human mitochondrial outer membrane protein VDAC2 (voltage-dependent anion channel) is a ?-barrel metabolite flux channel that is indispensable for cell survival. It is well established that physical forces imposed on a transmembrane protein by its surrounding lipid environment decides protein structure and stability. Yet, how the mitochondrial membrane and protein-lipid interplay together regulate hVDAC2 stability is unknown. Here, we combine experimental biophysical investigations of protein stability with all-atom molecular dynamics simulations to study the effect of the most abundant mitochondrial phosphocholine (PC) lipids on hVDAC2.